A PCR assay for detection of acetic acid-tolerant lactic acid bacteria in acidic food products

A PCR assay for the detection of acetic acid-tolerant lactic acid bacteria in the genera of Lactobacillus and Pediococcus was developed in this study. Primers targeting the bacterial 16S rRNA gene were newly designed and used in this PCR assay. To determine the specificity of the assay, 56 different bacterial strains (of 33 genera), 2 fungi, 3 animals, and 4 plants were tested. Results were positive for most tested bacterial members of 16S rRNA gene-based phylogenetic groups (classified in the Lactobacillus casei and Pediococcus group), including Lactobacillus fructivorans, Lactobacillus brevis, Lactobacillus buchneri, Lactobacillus plantarum, and Lactobacillus paracasei. For all other bacterial strains and eukaryote tested, results were negative. Bacterial DNA for PCR was prepared with a simple procedure with the use of Chelex 100 resin from culture after growth in deMan Rogosa Sharpe broth (pH 6.0). To test this PCR assay for the monitoring of the acetic acid-tolerant lactic acid bacteria, L. fructivorans was inoculated into several acidic food as an indicator. Before the PCR, the inoculation of 10 to 50 CFU of bacteria per g of food was followed by a 28-h enrichment culture step, and the PCR assay allowed the detection of bacterial cells. Including the enrichment culture step, the entire PCR detection process can be completed within 30 h.     

Suppressing the effect of lightning-induced overvoltages by the combined use of surge arresters and overhead ground wires

Lightning voltage induced by nearby strokes is one of the causes of major overvoltages which threaten the insulation of power distribution lines. Surge arresters as well as an overhead ground wire usually are employed for the protection of equipment and line insulation on overhead power distribution lines. The fundamental mechanism of suppressing effects of overvoltages induced by nearby strokes has been demonstrated independently by one of the present authors for surge arresters and for an overhead ground wire. In this paper, the protective effect of the combination of surge arresters and overhead ground wire is analyzed. As a result, it is found that the effect of surge arresters used together with an overhead ground wire is almost the same as that of surge arresters alone.     

Genomic organization and regulation of expression of the lectin-like oxidized low-density lipoprotein receptor (LOX-1) gene

Lectin-like oxidized low-density lipoprotein receptor (LOX-1) is a recently identified receptor for oxidized low-density lipoprotein, one of the major atherogenic substances. Although LOX-1 was reported to be expressed abundantly in endothelial cells, including atheromatous lesions, the regulation of LOX-1 gene has not yet been clarified. In the present study, we isolated the rat LOX-1 gene and investigated the regulation of gene expression. The rat LOX-1 gene was encoded by a single copy gene spanning over 19 kilobases and consisted of eight exons. Exon boundaries correlated well with the functional domain boundaries of the receptor protein. The promoter region contained putative TATA and CAAT boxes and multiple cis-elements such as NF-kappaB, AP-1 and AP-2 sites, and a shear stress response element. Northern blot analysis revealed that LOX-1 gene expression was up-regulated 9-fold by shear stress, 21-fold by lipopolysaccharide, and 4-fold by tumor necrosis factor-alpha, in cultured vascular endothelial cells. LOX-1 was also expressed in macrophages but not in vascular smooth muscle cells. These data provide important information for elucidating the molecular mechanisms of LOX-1 gene regulation and suggest a role for LOX-1 in the pathophysiology of atherosclerotic cardiovascular disease.     

AIGaAsSb Buffer/Barrier on GaAs substrate for InAs channel devices with high electron mobility and practical reliability

InAs/AlGaAsSb deep quantum well was successfully formed on GaAs substrate and examined for two electron devices, Hall elements (HEs), and field-effect transistors (FETs). With a thin buffer layer of 600 nm AIGaAsSb on GaAs substrate, we observed high electron mobility more than 23000 cm²/Vs and extrinsic effective electron velocity of 2.2 x 10⁷ cm/s for a 15 nm thick InAs channel at room temperature. AIGaAsSb lattice matched to InAs was discussed from the view points of insulating property, carrier confinement, and oxidization rate. Reliability data good enough for practical use were also obtained for HEs. We demonstrated AIGaAsSb as a promising buffer/barrier layers for InAs channel devices on GaAs substrate, and we discussed the possible advantages of AIGaAsSb also for InGaAs FETs.     

Decomposition of Benzene Using Ag/TiO2 Packed Plasma-Driven Catalyst Reactor: Influence of Electrode Configuration and Ag-Loading Amount

This paper describes the effects of electrode configuration and the loading amount of Ag catalyst on the decomposition of gas-phase benzene using plasma-driven catalyst (PDC) reactors. Modification of ground electrode brought out a great enhancement in the energy efficiency for benzene decomposition by reducing abnormal discharges outside the reactor tube. The data of carbon balance and the selectivity of CO₂ indicated that the Ag catalyst played an important role in the decomposition of benzene, especially for the intermediates. The larger the Ag-loading amounts on the TiO₂, the better the performance of benzene decomposition in terms of the carbon balance and the selectivity of CO₂. Formation of NO₂ and N₂O indicated that the maximum specific input energy applicable to the PDC reactor should be determined not only by the decomposition efficiency but also by the formation of nitrogen oxides.     

Creation of functional materials by use of a slow crystallization process in the nonequilibrium state. I. porous polyamide microparticles

Recently a number of investigations have focused on the self‐organization process from the nonequilibrium state as a new technique that may be used to develop many functional materials. In many cases, amorphous polymers were used and semicrystalline polymers were seldom used in spite of their importance. In this study, we basically investigated the crystal structure, crystalline process, and inner structures of polyamide by using slow phase separation and crystallization process from the nonequilibrium state of the polymer solution. We were able to observe the crystalline lamella growing twisted from the center of the particle. Between these lamella layers, narrow pores were created. From this investigation, we developed a new method to create functional materials of polyamide, the semicrystalline polymer. Porous spherical particles may be properly applied to functional materials such as adsorption materials, catalyst support materials, and so on. © 2003 Wiley Periodicals, Inc. J Appl Polym Sci 90: 2428–2432, 2003     

Fiber fuse phenomenon in triangular-profile single-mode optical fibers

The unsteady-state thermal conduction processes in triangular-profile (TP) optical fibers, which exhibited zero chromatic dispersion near 1.55 /spl mu/m, were studied theoretically with the explicit finite-difference method (FDM). It was estimated that these fibers would exhibit a high-temperature optical absorption on the basis of the high-temperature loss-increase mechanism proposed for step-index (SI) optical fibers. The core-center temperature of the TP fibers changed suddenly and reached over 7/spl times/10/sup 5/ K when a 1.064-/spl mu/m laser power of 1 W was inputted into the core layer heated at 2608 K. This rapid heating of the core initiated the "fiber fuse" phenomenon. The propagation rates of the fiber fuse, estimated at 1.064 /spl mu/m, were in fairly good agreement with the experimentally determined values. It was found that the threshold powers for initiating the fiber fuse are linearly proportional to the roots of the effective core areas of both the SI and the TP optical fibers. This coincides the experimental result reported by Seo et al.     

Effects of highly hydrogenated soybean oil and cholesterol on plasma,liver cholesterol,and fecal steroids in rats

We investigated the relationship between dietary highly hydrogenated soybean oil (HSO) and cholesterol transport in rats. In the first study, to examine the effects of cholesterol transport of different concentrations of HSO in dietary oil, rats were given one of the three diets containing 0, 25, or 50% HSO in dietary oil with cholesterol (5 g/kg diet) or a diet without HSO and cholesterol for 22 d. Feeding the high concentration of HSO prevented the increase in plasma total cholesterol, hepatic total lipids, and cholesterol and the decrease in high-density lipoprotein-cholesterol, which were caused by dietary cholesterol. Moreover, HSO increased the fecal excretion, fecal lipids, and steroids in a dose-dependent manner. In the second study, to examine the effects on cholesterol transport of redistribution of stearic acid in the triacylglycerol species contained in HSO, rats were given one of the six diets containing HSO (distearoylmonoacylglycerol and tristearoylglycerol)-rich, monostearoylglycerol-rich, or palmitic acid-rich oil with/without cholesterol (5 g/kg diet), for 30 d. Whereas the accumulation of cholesterol in the body was reduced, cholesterol excretion was enhanced effectively in rats given the HSO-rich diet compared with rats given the monostearoylglycerol-rich diet. These results suggested that not only the high concentration of stearic acid but also its uneven distribution in HSO-triacylglycerol contributed to the reduction in intestinal cholesterol absorption in rats.     

Simulation of fiber fuse phenomenon in single-mode optical fibers

The unsteady-state thermal conduction process in single-mode (SM) optical fiber was studied theoretically with the explicit finite-difference method (FDM). In the numerical calculation it was assumed that the electrical conductivity of the core layer increased rapidly above 1323 K. The core-center temperature changed suddenly and reached over 3/spl times/10/sup 5/ K when an optical power of 1 W was input into the core layer heated at 1373 K. This rapid heating of the core initiated the "fiber fuse" phenomenon. The high-temperature core areas, whose radiation spectrum extended over a wide range from the infrared to the ultraviolet regions, were enlarged and propagated toward the light source at a rate of about 0.7 m s/sup -1/. This rate was in fair agreement with the experimentally determined value.     

Fiber fuse phenomenon in step-index single-mode optical fibers

The unsteady-state thermal conduction process in step-index single-mode (SM) optical fiber was studied theoretically with the explicit finite-difference method. We considered a high-temperature loss-increase mechanism, which includes two factors that bring about an increase in the absorption coefficients: 1) electronic conductivity due to the thermal ionization of a Ge-doped silica core and 2) thermochemical SiO production in silica glass. The core-center temperature changed suddenly and reached over 4/spl times/10/sup 5/ K when a 1.064-/spl mu/m laser power of 2 W was input into the core layer heated at 2723 K. This rapid heating of the core initiated the "fiber fuse" phenomenon. The high-temperature core areas were enlarged and propagated toward the light source. The propagation rates of the fiber fuse, estimated at 1.064 and 1.48 /spl mu/m, were in fair agreement with the experimentally determined values. We found that the threshold power for initiating the fiber fuse increases from 0.98 to 1.26 W when the input laser wavelength is increased from 1.06 to 1.55 /spl mu/m.